Rapid conditional knock-down–knock-in system for mammalian cells
نویسندگان
چکیده
منابع مشابه
Rapid conditional knock-down–knock-in system for mammalian cells
RNA interference (RNAi) is a powerful tool to analyze gene function in mammalian cells. However, the interpretation of RNAi knock-down phenotypes can be hampered by off-target effects or compound phenotypes, as many proteins combine multiple functions within one molecule and coordinate the assembly of multimolecular complexes. Replacing the endogenous protein with ectopic wild-type or mutant fo...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2006
ISSN: 1362-4962,0305-1048
DOI: 10.1093/nar/gkl1055